Journal: International Journal of Molecular Sciences

Article Title: Targeted Suicide Gene Therapy with Retroviral Replicating Vectors for Experimental Canine Cancers

doi: 10.3390/ijms25052657

Figure Lengend Snippet: Replication kinetics of RRVs in canine cells. Normal canine cells (fibroblasts and primary liver epithelial cells) and canine tumor cell lines (AZACF, AZACH, AZACL1, AZACL2, AZACB, AZACU, oSCC-1, oSCC-4, TSCCLN#1, and TSCCLN#4) were infected with GFP-expressing RRV (AMLV or GALV) at a multiplicity of infection of 0.01. Cell growth was maintained and the percentage of cells expressing GFP was analyzed with a flow cytometer at every passage. Representative data from three independent experiments are shown.

Article Snippet: Normal canine liver epithelial cells were purchased from Cell Biologics, Inc. (Chicago, IL, USA).

Techniques: Infection, Expressing, Flow Cytometry

Journal: International Journal of Molecular Sciences

Article Title: Targeted Suicide Gene Therapy with Retroviral Replicating Vectors for Experimental Canine Cancers

doi: 10.3390/ijms25052657

Figure Lengend Snippet: Suicide-gene-mediated cell-killing effects in canine cells. Fibroblasts, liver epithelial cells, and AZACF, AZACH, AZACL2, oSCC-4, and TSCCLN#4 cells were infected with CD-expressing RRVs (AMLV or GALV) at an MOI of 0.01. On day 15, the cells were incubated with various concentrations of 5-FC for 6 days, and then cell viability was evaluated with an alamarBlue assay. Data shown are means ± SEs of triplicate experiments.

Article Snippet: Normal canine liver epithelial cells were purchased from Cell Biologics, Inc. (Chicago, IL, USA).

Techniques: Infection, Expressing, Incubation, Alamar Blue Assay

Journal: International Journal of Molecular Sciences

Article Title: Targeted Suicide Gene Therapy with Retroviral Replicating Vectors for Experimental Canine Cancers

doi: 10.3390/ijms25052657

Figure Lengend Snippet: List of cells used.

Article Snippet: Normal canine liver epithelial cells were purchased from Cell Biologics, Inc. (Chicago, IL, USA).

Techniques:

Journal: Oncoimmunology

Article Title: Overcoming the resistance of hepatocellular carcinoma to PD-1/PD-L1 inhibitor and the resultant immunosuppression by CD38 siRNA-loaded extracellular vesicles

doi: 10.1080/2162402X.2022.2152635

Figure Lengend Snippet: EVs/siCD38 retards tumor growth in mice . A, CD38 expression in mouse HCC Hepa1-6 cells and mouse liver epithelial cells BNL 1ME A.7 R.1 detected by RT-qPCR and Western blot analysis. B, Tumor volume of EVs/siCD38-treated mice. C, Tumor weight of EVs/siCD38-treated mice. D, IHC of CD38 and Ki-67 proteins in the tumor tissues of EVs/siCD38-treated mice. E, Adenosine content in tumor tissues of EVs/siCD38-treated mice. F, Macrophage polarization in tumor tissues of EVs/siCD38-treated mice by flow cytometry, scale bar: 50 μm. n = 6, * p < .05. The data in the figure are all measurement data, and are expressed as mean ± standard deviation. Comparison of data in two groups was conducted by independent sample t test. Data at different time points was analyzed by repeated measures ANOVA.

Article Snippet: Then, mouse liver epithelial cell line BNL 1ME A.7 R.1 (CL-0323, Procell), and mouse HCC cell line Hepa1-6 (SCSP-512, Cell Bank of Shanghai Chinese Academy of Sciences) were cultured in DMEM (PM150210, Procell) containing 10% FBS, 100 U/mL penicillin and 100 U/mL streptomycin.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Flow Cytometry, Standard Deviation, Comparison